The AtheNA Multi-Lyte® Test System is a breakthrough in
multiple-analyte testing. It is a multiplexed system utilizing the
patented Luminex® xMAP® technology. Polystyrene microspheres of
uniform size are used as the solid phase.
Each bead set is treated with a unique combination of
two fluorescent dyes and conjugated to a different antigen, making
it possible for the system to identify unique bead sets.
Thousands of each bead set are combined to form a multiplex bead
suspension. The bead suspension is added to the wells of a
microplate.
Unique bead sets can be conjugated with various, unique
target molecules of interest.
If antibody from the test sera is present, it will bind to the
antigen-coated bead. Anti-human Ig reporter "tags" then bind with
the antibody.
If the patient possesses antibody to more than one bead set, all
beads will be labeled with antibody and then conjugate. The bead
suspension is analyzed using the AtheNA instrument.
Beads flow through the flow cell and light scatter will
determine discrete events.
One laser identifies the amount of reporter on the surface. The
other determines the amount of classification dyes... or identifies
the bead set.
This flow analysis of the beads occurs at a rate of up to 20,000
beads per second. Results are based on 50 individual bead
reactions, the equivalent of running each sample 50 times in 50
separate EIA/IFA wells.
View How It Works (PowerPoint
Presentation)